Enzymatic method for ascorbic acid determination
- Kit volume: 90 ml
- Method: Two-reagent differential at 560 nm
- Limit of linearity: 150mg/L
- Limit of detection: 1.4 mg/L
Advantages
- Stable working reagent for 10 days
- Ready-to-use dedicated reagent
- Calibrator included in the kit. Once reconstituted, stable for 20 days
Ascorbic acid is a compound found in ripe grapes at very low levels compared with other acids (30-60 mg/L). It disappears rapidly when grapes are crushed, leading to early oxidation of must. Due to its reducing properties, ascorbic acid is used as an antioxidant.
Ascorbic acid in the sample lowers MTT in the presence of PMS electron carrier, forming dehydroascorbic acid and MTT-formazan that can be assayed by spectrophotometry. To eliminate interferences ascorbic acid is eliminated from the sample by oxidation to dehydroascorbic acid (ascorbate oxidase [AO]).
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