Colorimetric analysis for total sulfite determination
- Kit volume: 200 ml
- Method: Two-reagent differential at 405 nm
- Limit of linearity: 400 mg/l
- Limit of detection: 1 mg/l (ppm)
Advantages
- Stable liquid reagent until the expiration date
- Ready-to-use dedicated reagent
- Liquid calibrator included in the kit
Sulfite is the main preservative of wines and musts, due to its antiseptic properties on yeasts and bacteria; it also has antioxidant properties. According to Council Regulation (EC) No 1493/1999 and Council Regulation (EC) No 1622/2000, the sulfur dioxide content of wine is limited, as it is considered to be a slightly toxic substance from the point of view of its effects on human physiology.
Total sulfites in the sample react with 5-5’-dithio-2-nitroben- zoic (DTNB) acid in basic medium. Cleavage of the disulfide bond (R-S-S-R) of DTNB by a sulfite molecule yields the 5-mercaptan- 2-nitrobenzoate molecule, which absorbs at 405 nm. The color increase of the sample is directly proportional to the total sulfite concentration of the sample.
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