Primary Amino Nitrogen (PAN)

Colorimetric analysis for primary amino nitrogen determination

• Kit volume: 100 ml
• Method: Two-reagent differential at 340 nm
• Limit of linearity: 400 mg/l
• Limit of detection: 1 mg/l (ppm)

Advantages

• Stable liquid reagent until the expiration date
• Stable working reagent for 12 months
• Ready-to-use dedicated reagent
  
• Liquid calibrator included in the kit

Nitrogen compounds (molecules containing a primary amine nitrogen) in must and wine play a key role in fermentation and the potential of microbial stability.

Any molecules in the sample that contain a primary amino nitrogen react with o-phthaldialdehyde (OPA) in the presence of a reducing agent in basic medium, yielding a chromogen that is assayed spectrophotometrically.

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