Enzymatic method for D-glucose / D-fructose determination
- Kit volume: 120 ml
- Method: Two-reagent differential at 340 nm
- Limit of linearity: 8 g/l
- Limit of detection: D-Glucose: 0.03 g/l. D-Glucose / D-Fructose: 0.02 g/l
Advantages
- Stable liquid reagent until the expiration date
- Ready-to-use dedicated reagent
- Working reagent stable until the expiration date
- Liquid calibrator included in the kit
This test indicates the best moment for grape harvesting and allows alcoholic fermentation to be monitored. It is widely used to determine the dryness of the wine before bottling.
D-fructose and D-glucose in the sample generate NADH (by the following reaction), which can be measured by spectrophotometry. The configuration of these reagents allows D-glucose/D-fructose (total sugars) to be determined if the enzyme PGI is added or D-glucose to be determined if it is not.
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